The Application of Radio-Frequency Impedance (RFI) in Cell Culture

radio frequency impedance rfi

NEED FOR THE ABER RFI MEASUREMENT IN CELL CULTURE Article republished with permission from Aber Instruments. Download PDF of this case study here. There is an increased focus on in‐line techniques for bioprocess monitoring driven primarily by the initiative of the FDA regarding “Process Analytical Technology” (PAT). Traditionally, parameters such as pH, dissolved oxygen and […]

OD600 Measurement Results Using Spectrophotometers & Online OD Instruments

Spectrophotometers can be an excellent tools to monitor the growth phase or determine cell concentrations in microbial fermentation processes. Despite of the fact that these instruments are generally utilized to measure absorbance of particular samples (concentration measurements), spectrophotometers are also used to quantify light scattering running at 600 nm wavelength. This method, commonly known as […]

PAT Solutions Provider PROAnalytics Welcomes Brian Trippe as Senior Sales Engineer

PROAnalytics welcomes Brian Trippe

New hire’s years of technical experience help expand company’s customer base and product offerings Fairfield, NJ, Dec. 6, 2018 – Bioprocess, process analytical technology, and laboratory equipment supplier PROAnalytics, LLC is pleased to announce that Brian Trippe has joined the company as Senior Sales Engineer and Product Specialist. Brian will be responsible for developing new […]

Density Measurement: Best Operating Practices for the Laboratory

The latest advances in digital technologies allow users to perform a density measurement with a high degree of precision and great resolution. To maximize the accuracy of results, however, users may benefit by applying the best operating practices described in this article. Advantages of Digital Automatic Density Measurement Digital density meters typically use electromagnetically-induced oscillation […]

Coulometric Titration vs Volumetric Titration: Using KF titrators to determine water content

The Karl Fisher titration method was published to determine trace amount of water samples in non-aqueous systems that contained sulfur dioxide. It is currently the standard method used to calculate the moisture content in various products. When looking at titration analysis methods, it can be a challenge to determine which to use. This article takes […]

Introduction to UV/VIS Spectrophotometry: Using Spectrophotometer To Determine Concentration

Ultraviolet and visible light range (UV/VIS) is widely applied in research, production and quality control for the classification and study of substances. UV/VIS spectroscopy is based on the absorption of light by a sample. Depending on the amount of light and its wavelength absorbed by the sample, valuable information can be obtained, such as the purity […]

Continuous Glucose / Lactate Analyzer for Fermentation Monitoring & Control

Production of biologics based on Recombinant DNA technologies through mammalian cell culture / bacterial fermentation require precise monitoring and control of key process parameters. The glucose concentration – main carbon source – is one of the most important ones. A method for online glucose measurement has been applied and perfected during the last 15 years […]

Photometric Analysis Performance Advantages in Upstream Cell Culture Operations

Recombinant Therapeutic Proteins development and production operations require reliable and accurate biochemistry analyzers to characterize the cell culture process and maximize results through photometric analysis. Traditional membrane based (electrochemical) analyzers still in use in many labs present multiple problems including measurement drift and the need of high frequency maintenance. An advanced, more accurate technology based […]

DO & pH Probe Calibration for Optimized Measurement Results in Biopharma

pH and Dissolved Oxygen are critical parameters in bio fermentation and cell culture applications. The proper selection of probes in construction, quality and design is essential. However, to maximize the accuracy of results, it is equally important to utilize the right DO and pH probe calibration and maintenance techniques. pH Probe Calibration Techniques: Prior to […]

Metabolite analysis in cell culture – Photometric analyzer vs membrane-based systems

Optimization of Glutamine Measurement in Cell Culture Operations Current “membrane based” instrumentation utilized at a large cell culture manufacturing site has presented recurrent problems including non-repeatable, inaccurate metabolite analysis, especially of Glutamine. These membrane / electrochemical technologies have shown inherent flaws in measurement stability and excessive maintenance at multiple sites. Newer optical-based metabolite analyzers were evaluated to […]