Optimization of Glutamine Measurement in Cell Culture Operations Current “membrane-based” instrumentation utilized at a large cell culture manufacturing site has presented recurrent problems, including non-repeatable, inaccurate metabolite analysis, especially of Glutamine. These membrane / electrochemical technologies have shown inherent flaws in measurement stability and excessive maintenance at multiple sites. Newer optical-based metabolite analyzers were evaluated to determine […]

Authors: Holly Sullivan, David Koechlein, Justin Lussier Introduction The ability produce biological drugs at lower costs and higher yields has been a major focus for biomanufacturing. With the advent of Single-Use Bioreactors and improved molecular biology, cell cultures can be > 20 x 106 cells/mL (1). Clarification of these cultures typically involves a primary depth […]