PAT Symposium

Inline Sensors & Automation Tools Enabling Advanced Bioprocess Control

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Join us for a FREE networking & learning event with PAT industry pros, end users, and partners.

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Thought Leaders

Our Speakers

Alexis (Lexi) Dubs

PhD Candidate, MIT

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An Zhang

Principal Engineer, Biogen

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Shaun Fraser

Lead Scientist, Tornado, a Bruker Company

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Innovations in PAT

Why Attend This Complimentary Symposium?

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Discover how PAT technologies are being utilized in upstream applications across scales from R&D to Production for enhanced efficiency and innovation in bioprocessing.

Behind The Bioprocess

Program Agenda

Join us for this half-day event to network with industry professionals, end users, and partners at this exciting symposium near Kendall Square in Cambridge, MA.

This event and setting provide a unique opportunity to engage closely with leading experts who will share cutting-edge insights and present compelling case studies designed to facilitate collaborative discussions and exchanges of ideas.

There will also be hors d’oeuvres and drinks served with an opportunity to mingle with each other and interact “hands-on” with the equipment!

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Introduction and Case Studies

1 pm – 3:30 pm

Networking and Hands-On Tech

3:30 pm – 5 pm
Empowering Bioprocessing

Technologies On-Site

Aber Instruments is bringing Optura and Futura instrumentation to support biomass monitoring. Tornado (a Bruker Company) will have their HyperFlux PRO Plus and several Raman probes on hand. ProAnalytics will showcase Securecell’s Numera autosampler alongside their bioprocess software, Lucullus.

Case studies from Bioprocessing Experts brought to you by

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The Program

Session Details

Testbed Development for a mAb Production Process

Biologic products, including mAbs, are structurally complex with several critical quality attributes (CQAs) that can affect immunogenicity, product stability, and clearance rates from the body. These product CQAs are directly related to processing conditions, and thus control of the process conditions can enable control of the CQAs. In order to better understand these relationships, it is important to have process and quality data. In this presentation, we describe the development of a testbed to generate this data and discuss how the Lucullus software can be used as a DCS. We also discuss how we can use the Lucullus REST API to integrate this DCS with the sophisticated codes needed to perform model based control.

From Innovation to Application: PAT Development Roadmap in Cell Culture Manufacturing Process

Various PAT technologies have been emerging over the past several decades to enhance the process productivity and robustness in biopharmaceutical manufacturing. However, their adoption hurdles have remained in the highly regulated bioprocesses. In this case study, we demonstrated a roadmap of developing an on-line monitoring and control of cell culture process using dielectric spectroscopy from lab scale to GMP manufacturing.

Upstream and Downstream PAT with HTVS-powered Raman

Raman spectroscopy is an important Process Analytical technology (PAT) tool in the realm of biopharmaceutical manufacturing, facilitating real-time monitoring and optimization across both upstream and downstream processing stages. Raman spectroscopy has often been used for monitoring metabolic substrates and waste products, such as glucose and lactate. Using Raman spectroscopy for the continuous monitoring of critical parameters such as glucose and lactate levels has been extensively demonstrated in literature and has traditionally used several batches to develop the chemometric models needed for monitoring. Tornado Spectral Systems – A Bruker Company, aims to demonstrate the integration of plug-and-play and semi-plug-and-play models for their efficacy in minimizing offline data requirements and accelerating model development, thereby enhancing process control and efficiency.

Raman spectroscopy also has great applicability in downstream processing, where the molecular specificity of Raman signals enables the real-time monitoring of individual proteins and other materials. This specificity proves instrumental in affinity chromatography, where precise protein purification protocols are essential for optimizing bioprocess efficiency. Raman has been traditionally limited in its applications in BioPharma downstream due to slow measurement times. The High Throughput Virtual SlitTM (HTVSTM) technology allows for more than 10x greater signal throughput, enabling Raman to be more effectively applied in the downstream space. Experimental data on Raman monitoring of breakthrough detection in affinity chromatography is demonstrated, showing the ability of Raman to track a target protein (Human Serum Albumin) in the presence of confounding proteins (lysozyme).

Through its ability to provide real-time, molecular-level insights, Raman spectroscopy facilitates the advancement of bioprocess monitoring and optimization, ultimately contributing to the production of high-quality biopharmaceuticals.


Revolutionize Bioprocessing

Join Us in Cambridge

May 16, 2024 from 1pm–5pm ET at the Residence Inn at Boston Cambridge

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