Multimini pH/DO calibration interface and WebUI main interface

Parallel Bioreactor Systems

AbioBundle Multimini™ by Applitech is a miniature multi-parallel bioreactor designed for high-throughput process development, optimization, and scale-up studies. Available in a standard 4-vessel configuration and scalable to as many as 32 parallel vessels, the system supports both mammalian cell culture and microbial fermentation applications.

With working volumes from 50 to 400 mL, the Multimini provides a compact parallel bioreactor system for scale-down studies while maintaining geometric similarity to larger Applitech bioreactors for transfer to pilot- and production-scale processes.

Standard 4-vessel configuration, scalable to 32 parallel bioreactors
50–400 mL working volume
Independent or synchronized vessel operation
Supports mammalian cell culture and microbial fermentation
Geometrically scalable vessel design for scale-down and scale-up studies
Designed for high-throughput process development

Applications

Design of Experiments (DOE)
Media optimization
Clone screening
Process characterization and optimization
Mammalian cell culture
Microbial fermentation
Recombinant protein production
Cell and gene therapy process development
Enzymatic and cell-free reactions
In vitro RNA transcription

Specifications

Feature	Cell Culture	Microbial Fermentation
Vessel number	Standard 4 vessels, scalable to 32-parallel control	Standard 4 vessels, scalable to 32-parallel control
Software	AppliSoftec Lite (standard), Advanced version optional	AppliSoftec Lite (standard), Advanced version optional
Vessel type	Glass	Glass
Working volume	50-200mL/100-400mL	50-200mL/100-400mL
Agitation	Top mechanical drive coupling	Top mechanical drive coupling
Impeller	Standard Marine, customizable	Standard Rushton, customizable
Temperature control mode	Peltier-based water-free temperature control	Peltier-based water-free temperature control
Temperature range	RT -10°C to RT +30°C	RT -10°C to RT +30°C
Feeding	4 standard feed ports	4 standard feed ports
Feed bottles	15/50 mL disposable feed bottles (standard)	15/50 mL disposable feed bottles (standard)
Peristaltic pumps	Up to 3 pumps, max speed 150 rpm	Up to 3 pumps, max speed 150 rpm
Gas control	MFC, headspace and sparging (macro/micro bubbles)	MFC, bottom sparging (macro bubbles)
Gas flow rate	Optional range	Optional range
pH	CO2/base solution, customizable	Acid/base solution, customizable
DO	PID control with proprietary Applitech algorithm	PID control or Gradation Control
Antifoam / Level	Optional	Optional
Off-gas condenser	Optional Peltier cooling	Optional Peltier cooling
Motor / electrode holder	Standard	Standard